It has been demonstrated that virus‐triggered Ca2+ influx plays essential roles for viral entry and replication.[14, 41, 42, 43, 44] A number of FDA‐approved drugs have been screened to target intracellular Ca2+ or Ca2+ channels for inhibiting viral infections.[10, 45, 46, 47] Consistent with this notion, we observed that pharmacological inhibition of TRPV2 by SKF96365 in Trpv2fl/fl BMDCs and BMDMs or reconstitution of TRPV2E572Q into Lyz2‐Cre;Trpv2fl/fl BMDCs inhibited the penetration of HSV‐1. Here, TRPV2 is linked to viral infectious disease.