To determine whether the calcium permeability was sufficient to restore virus penetration, we reconstituted wild‐type TRPV1 or TRPV1D646N/E648/651Q into Lyz2‐Cre;Trpv2fl/fl BMDCs followed by infection with VSV or quantum dots‐encapsulated SV40 viruses and analysis with qRT‐PCR or HiS‐SIM live cell imaging. Here, TRPV1 is linked to infection.