ACTA1 and metabolic dysfunction-associated steatotic liver disease: In contrast, immunohistochemical markers of inflammation (CD11b), macrophage infiltration (CD45, Galectin-3) and fibrogenesis (COL1A1, αSMA) were not significantly elevated in individuals with moderate or more advanced NAFLD as compared to individuals with no/mild NAFLD (S2 and S3 Figs).