It was observed that the growth rate of xenografts in nude mice in the NC group treated with PBS was the fastest, and the growth rate of xenografts slowed down, the tumor volume and weight was reduced after the application of HMGB1 silencing, especially combined with pathway inhibitor ly294002, and the trend was more obvious after irradiation (Fig. 4A). This evidence concerns the gene HMGB1 and neoplasm.