CD80 and neoplasm: 2019). Again, data are scarce. Recently, we used double immunohistochemical staining to study the spatial distribution of CD8 + T cells in relation to B7–H3 positive cells. B7–H3 was expressed mainly in the tumor stroma of GC (76% of all cases). GCs with high expression of B7–H3 showed larger spatial differences of CD8 + T cells (86.4/mm2 in tumor center vs. 414.9/mm2 in invasive front) compared to the B7–H3-low group (157.7/mm2 vs. 218.7/mm2, respectively) (Ulase et al. 2021).