FOXI3 and microtia: This included an additional missense variant in the NLS (p.Arg240Cys) in a kindred with isolated microtia that was predicted to be deleterious (Combined Annotation Dependent Depletion score = 29.1, Polymorphism Phenotyping v2 score = 1.00) and similarly resulted in the disruption of FOXI3 nuclear localization in vitro (Figure 1C and D).