AFP and hepatocellular carcinoma: In another study on the relationship between preoperative serum AFP level and prognosis of HCC patients, 20 ng/mL and 400 ng/mL were selected as cut-off values, and the results showed that preoperative serum AFP level has considerable predictive value for the malignant feature and a preoperative AFP value >400 ng/mL was independent prognostic factors for long-term survival and associated with poor prognosis after resection for HCC [13].