However, when we focused on the top 500 genes with the lowest loadings along the disease model axis (corresponding to a reduced expression in AD models), we found more AD GWAS loci genes than expected by chance (hypergeometric test, P=0.00197), including HBEGF, CASS4, OARD1, CNN2, IL6R, BZW2, BIN1, FRMD4A and ADAM10 (Fig. S12D). Here, CASS4 is linked to Alzheimer disease.