Chi-square analysis showed that ITGA4 was mainly related to T classification (P = 0.028), N classification (P = 0.006), lymph node metastasis rate (P = 0.018), and serum CA19-9 (P = 0.003) (Table 2), which was basically consistent with the results of previous transcriptome level analysis. This evidence concerns the gene ITGA4 and metastatic malignant neoplasm in the lymph nodes.