Moreover, a significant changes of several cytokines related to Th9 cell differentiation in mouse asthma model was detected compared with the control mice group, including forkhead box O1(FOXO1), IL-4, IL-9, IRF4, Spi1, BATF, and IRF1 [2, 4], (Additional file 1: Fig. S1). Here, IL4 is linked to asthma.