SIRT5 and colorectal carcinoma: As anticipated, the expression of γH2AX, a marker of DNA damage, was upregulated in multiple human CRC cell lines (HCT116, LoVo, and HT29) after treatment with two short-interfering RNAs (siRNAs) targeted at SIRT5 for 24, 36, 48, and 72 h (Fig. 1a and Supplementary Fig. 1a).