SIRT5 and colorectal carcinoma: Considering that SIRT5 is often overexpressed in CRC cells, we constructed a control vector, a SIRT5 wild-type plasmid (SIRT5 WT), and a H158Y mutant plasmid (SIRT5 H158Y, a catalytically inactive mutant without lysine deacylation activity), and reintroduced them into CRC cells.