To extend the physiological relevance of our results, we examined the functional roles of NHE1 and/or SWELL1 in cell dissemination from 3D breast cancer spheroids embedded in 3D collagen gels (Fig. 1g; Supplementary Movie 3) or on 2D collagen I-coated surfaces (Supplementary Movie 4). Here, LRRC8A is linked to breast carcinoma.