Importantly, 3D CRC organoid culture validated the decreased H3K27ac signal at the PDZK1IP1 (log2 fold change −0.48, P = 0.05) and IFITM1 (log2 fold change −1.87, P = 0.0002) SEs observed in 2D culture (Fig. S6e, f). This evidence concerns the gene PDZK1IP1 and colorectal carcinoma.