To further corroborate this observation, we evaluated 5hmC levels at binding sites of the key prostate cancer oncogenes AR, FOXA1 and HOXB13 and H3K27ac modifications (from mCRPC xenograft data), and observed that 5hmC was enriched at these loci in mCRPC samples (Fig. 2D), suggesting that 5hmC marks the reported cistrome reprogramming associated with activation of developmental programs (21). This evidence concerns the gene AR and prostate cancer.