CD40LG and substance abuse: Prenatal routine maternal testing was performed for toxoplasmosis, hepatitis B and C, human immunodeficiency virus, and syphilis. Maternal comorbidities, substance abuse and medications were recorded. Infants were subjected to testing for syphilis (treponemal and reaginic tests) and toxoplasmosis (IgM and IgG ELISA). Blood, saliva, urine, and/or liquor were tested for enterovirus, parvovirus, HHV-6, HSV-I, HSV-II, and CMV via PCR assays.