The study related to the use of CRISPR/Cas9 technology, for the knockout in DCLK1 is overexpressed and the overexpression of DCLK1 in less expressed BC cell lines, respectively, suggested that DCLK1 promotes cancer invasion and metastasis, which on downregulation shows reduced invasive and migration ability (Liu et al., 2019). The gene discussed is DCLK1; the disease is breast cancer.