To address whether frequencies of CD3+CD4+ TILs and CD3+CD8+ TILs and their activation or exhaustion state in vivo affect their autologous tumor-cell reactivity, we performed extensive phenotyping by flow cytometry of 15 of 17 bulk TIL cultures after CD3+ selection with Dynabeads; 2 bulk cultures could not be analyzed because of lack of sufficient cells. This evidence concerns the gene CD8A and neoplasm.