By day 12 postinoculation, the B16-tmCRT/39-272 cells appeared to be much more aggressive than the B16-EGFP control, as the earlier results showed, but there was no significant difference in the weight of the two TLR4-KO mouse groups (Figures 3(e) and 3(f)), indicating that tmCRT/39-272 promoted tumor malignancy through interaction with TLR4. The gene discussed is TLR4; the disease is neoplasm.