PTEN and glioblastoma: The established mouse glioblastoma GL261-EGFP cell line and mouse TRP-EGFP-Fluc cell line of transformed astrocytes (truncated SV40 T antigen, Kras G12D mutation, Pten deletion)32 were intracranially injected in the transgenic C57Bl/6 mice with wide-spread expression of tdTomato (RFP+).