Indeed, to determine the main stage (namely, the mechanisms) of autophagy that Huaier influenced in pancreatic cancer cells, we detected autophagic flux by using ad-mRFP-GFP-LC3, and the results showed that Huaier can increased the number of autophagosomes (yellow-labelled LC3); however, it has no effect on “smoothing” the process of autophagic flux (Figures 4E–G, no difference between on the level of red-labelled LC3 reflect autophagolysosome in the control and Huaier groups), which reminds us Huaier-induced increase in autophagosomes may be partly due to accumulation effect. This evidence concerns the gene MAP1LC3A and familial pancreatic carcinoma.