Consistent with this, the IHC assay showed high levels of the early autophagy markers LC3 (Figure 4H) and BECLIN/ATG5 (data not shown) in the Huaier-treated pancreatic tumour group, but the level of late autophagy markers P62 did not differ between the control and Huaier groups (Figure 4H). This evidence concerns the gene MAP1LC3A and pancreatic neoplasm.