In the analysis of IDHWT GBM single-cell RNA-seq data, we further determined that macrophage-related MRGs have closer molecular interactions with other MRGs than other cells, and identified 5 MRGs (ABCA1, MTHFD2, HMOX1, PIM1, and PTPRE) that were significantly changed in TAMs upon switching from low to high risk. This evidence concerns the gene MTHFD2 and glioblastoma.