After knocking down NCAL1 (Figure 2A), the ability of NK cells to kill tumor cells decreased at different effector-to-target ratios (1.25:1, 29.91% vs. 14.14%, p < 0.001, 29.91% vs. 23.08%, p < 0.05; 2.5:1, 43.61% vs. 28.30%, p < 0.001, 43.61% vs. 36.60%, p < 0.01; 5:1, 55.07% vs. 36.81%, p < 0.001, 55.07% vs. 44.80%, p < 0.001) (Figure 2B). The gene discussed is NCAL1; the disease is neoplasm.