PRAME and neoplasm: The TAA/ABL1 mRNA ratios in the tumor cell line-spiked samples were highly reproducible, with a CV of 0.05 and 0.11 for WT1/ABL1, 0.1 and 0.21 for PRAME/ABL1, and 0.12 and 0.21 for BIRC5/ABL1 (Table 1).