The data detected by ELISA suggested that Tai Chi intervention for 12 weeks could significantly reduce the concentration of the serum inflammatory factors including ROS, NF-κB, NEK7, NLRP3, ASC, Caspase-1, GSDMD, IL-1β, and IL-18 in patients with pre-diabetes, indicating that Tai Chi intervention could relieve vascular and systemic inflammation. This evidence concerns the gene NLRP3 and diabetes mellitus.