To evaluate the global transcriptomic impacts of reduced HSP27 and TGF-β1 expression, various cancer cell lines, including A549, SNU-398, MDA-MB-231, and MIAPaCa-2, were treated with shRNAs targeting TGF-β1 (shTGF-β1) and HSP27 (shHSP27), either alone or together (shHSP27-shTGF-β1), and assessed by microarray analyses using 47,316 probe sets. This evidence concerns the gene TGFB1 and cancer.