Another study also showed that tRF-5 was abundant in the sperm of mice with myocardial hypertrophy and inhibited the mRNA expression of the hypertrophy regulator TIMP3 by binding its 3′UTR sequence, thereby aggravating cardiomyocyte hypertrophy, increasing cardiac fibrosis and promoting apoptosis (34). This evidence concerns the gene TIMP3 and cardiac hypertrophy.