A dose-dependent relationship with vasoconstriction was found after stimulation of endothelium-denuded rat intrapulmonary arteries by applying Yoda1, and Piezo1 activity was significantly upregulated and increased in idiopathic PAH-PASMCs compared with donor PASMCs, which also explains the effect of proliferation caused by elevated Piezo1-mediated [Ca2+] i in PASMCs under PAH on pulmonary artery of the molecular mechanisms of vascular remodeling. This evidence concerns the gene PIEZO1 and pulmonary arterial hypertension.