Therefore, in the cause of studying the effects of CLCN2 and CLCN6 expression on metabolic process, the Seahorse XF96 extracellular flux analyzer was applied to explore how downregulation of CLCN2 or CLCN6 affected glycolytic function and mitochondrial function in PCa cells. The gene discussed is CLCN6; the disease is posterior cortical atrophy.