It was determined that the FT4 levels were 15.63, 5.91, and 12.37 ng/ml, the E2 levels were 63.21, 46.29, and 57.03 ng/L, the FSH levels were 6.01, 3.70, and 4.83 IU/L, and the LH levels were 5.61, 2.51, and 4.38 mIU/ml in the Control-ER, Hypothyroid-ER and Treatment-ER groups, respectively, and all these differences were found to be statistically significant (P<0.001). The gene discussed is BRD2; the disease is hypothyroidism.