ACTA2 and neoplasm: A series of genes involved in epithelial‐mesenchymal transition (EMT) and tumour invasion and migration (Table S7) were expressed at higher levels in tumour areas of TLS‐negative ESCCs than TLS‐mature and TLS‐immature ESCCs, such as the mesenchymal markers FN1 (Fibronectin 1), ACTA2 and SNAI2, basement membrane components collagens, laminins, integrins and filamins,24 MMPs and so on (Figure 6A; Table S12).