ALDOB and glioblastoma: Furthermore, we observed that 2,5-AM treatment impaired the fructose-mediated colony formation of U87 cells under glucose-deprived condition, but this effect was not observed in U87 cells with knockdown of SLC2A5 or ALDOB (Supplementary Figs. 4f, 6c), suggesting that 2,5-AM treatment inhibits GBM cell survival depending on GLUT5 and ALDOB expression.