Immunohistochemical (IHC) analyses with anti-ATF4, anti-GLUT5, anti-ALDOB, anti-Ki67, anti-cleaved PARP1 (cPARP1), and anti-uH2B antibodies demonstrated that tumor tissues derived from U87 cells with ATF4 KO exhibited no expression of ATF4, low expression of GLUT5 and ALDOB, decreased expression of Ki67, and increased PARP1 cleavage (Supplementary Fig. 5c). This evidence concerns the gene ATF4 and neoplasm.