Based on the recent findings indicating that the N-terminus of AMBRA1 is involved in stabilization of AMBRA1 itself [30] and of Cyclin D1 [26–28], a result that we confirmed in BRAFV600E-mutated A375 melanoma cells silenced for AMBRA1 by small interference RNA (siRNA; siAMBRA1 #1 and #2) (Supplemental Fig. 1), we characterized the in vitro effects of AMBRA1 mutations (REVEL score ≥0.4) within those mapping at the N-terminus of the protein (Fig. 3A). The gene discussed is AMBRA1; the disease is melanoma.