ITGAM and neoplasm: To demonstrate the pro-inflammatory traits of TAMs with increased Ly6C-expression [49] as well as the tumour promoting characteristic of TAMs with reduced Ly6C-expression [50], we co-cultured the CD11b+Ly6C+ AMJ2-C11 syngeneic mouse macrophage cell line (Fig. S7A) with B16F10 cells for up to 21 days.