We performed a multiplex immunolabeling protocol using Opal fluorophores for the following set of markers: Col1, Cytokeratin (CK), CD45, aSMA, vWF, CD163 and DAPI (nuclear stain) (Fig. 6A) to identify cancer cells (Cytokeratin+CD45–), fibrocytes (CD45+Col1+CD163+), fibroblasts (aSMA+Col1+CD163–), macrophages (CD163+ Col1–) and endothelial cells (vWF+). The gene discussed is CD163; the disease is cancer.