Here, the 13 Clspn+/− mice had 23 HCCs in total from the liver sections analysed, including one mouse with 2 grade II and one mouse with 1 grade III HCC, versus only six grade I HCC in the WT mice (Figure 5E,F and Supplementary Figure S3F–H). This evidence concerns the gene CLSPN and hepatocellular carcinoma.