As an alternative approach, we sorted CD4+ T cells and CD8+ T cells from the spleens of tumor-bearing Cd4;Tcf7fl/fl and control Cd4-CreERT2;Tcf7+/+ mice (referred to as Cd4;Tcf7+/+; Fig. S2 B) and used quantitative RT-PCR (qRT-PCR) to measure the expression levels of Tcf7 and the WNT signaling target gene Axin2. Here, CD4 is linked to neoplasm.