In our experiment, SL reduced the percent apoptosis of cardiomyocytes, downregulated the expression of pro-apoptotic proteins (cleaved caspase-3, cleaved caspase-9, Bax), and upregulated the expression of an anti-apoptotic protein (Bcl-2) in MI/R mice, meanwhile, in the conditional medium transfer models, our results also showed that SL reduced H/R cardiomyocytes apoptosis and rate of cleaved-caspase-3/caspase-3 and raised Bcl2/Bax ratio, which suggested SL reduced cardiomyocyte apoptosis in MI/R mice and H/R cardiomyocytes induced by M1 macrophages. This evidence concerns the gene CASP3 and myocardial infarction.