Andrographolide combined with methotrexate has a certain effect on the treatment of CFA-induced arthritis and can significantly reduce the expression levels of proinflammatory cytokines (TNF-α, IL-6, and IL-1β) in serum, alleviate methotrexate-induced hepatocyte injury and enhance anti-inflammatory effects (Li et al., 2018a). This evidence concerns the gene IL6 and Arthritis.