CD274 and rectal cancer: In the 27 rectal cancer samples we collected and sequenced (DPH data set), there were no significant differences in cytotoxic T lymphocytes, PD-L1 expression, follicular regulatory T cells, and TLS indicators among non-chemoradiotherapy (uCRT), non-pathology complete remission (nPCR), and PCR groups (Figure 6), and only the FTBRS value was significantly higher in the PCR group than that in uCRT samples (adjusted P = 0.002).