The number of pThr509-CRMP1–positive spheroids per anterior horn area (mm2) in the ALS patient group was significantly higher than that in the disease control group (Figure 2F) and was negatively correlated with disease duration, but there was no correlation with age at death, initial symptoms, walking score of the ALS Functional Rating Scale–Revised, or the number of residual neurons (Supplementary Table S2). This evidence concerns the gene CRMP1 and amyotrophic lateral sclerosis.