CRMP1 and amyotrophic lateral sclerosis: While verifying the co-localization of pThr509-CRMP1 and pNFs in spheroids, we noticed that the immunofluorescence intensity of pThr509-CRMP1 was higher than that of pNFs in the spinal cord of an ALS patient with short disease duration (3 months), and vice versa in a patient with a long disease duration (48 months) (Figure 3A).