However, they were also enriched in various pathways that are important for cancer development, immune checkpoint, drug tolerance, and pluripotent differentiation of stem cells (e.g., P53 signaling pathway, HIF-1 signaling pathway, FOXO signaling pathway, specific to hotspot genes; PD-L1 expression and PD-1 checkpoint pathway in cancer, platinum drug resistance, signaling pathways regulating pluripotency of stem cells, specific to single-line regulated genes) (Figures 5C,D; Supplementary Table 4). The gene discussed is TP53; the disease is cancer.