HK2 and infection: Our results now show that HAdV-D36 infection of 3T3-L1 cells also results in increased levels of glycolytic Myc-target genes displaying a clear effect on Hk-2 and PFk, in agreement with previous findings (Thai et al., 2014; Kong et al., 2015) (Figure 7), suggesting that the HAdV-D36 E4Orf1 may stimulate glucose metabolism through Myc, in addition to its effect through PI3K.