Key findings of this study include 1) expression of bub1 was significantly related to the development and clinicopathological characteristics of neuroblastoma, 2) bub1 demonstrated a prognostic value for patient survival, 3) expression of bub1 was associated with host immune infiltration of neuroblastoma microenvironment, 4) bub1 exerted its biological function possibly by regulating the expression of proteins related to EMT, cell apoptosis and the Wnt signaling pathway. This evidence concerns the gene BUB1 and neuroblastoma.