On the other hand, analysis of TGFβ- mRNA expression showed (Figure 8c) the highest values in WOT at day 5, decreasing suddenly at day 7, while in the KC group, the TGF-β- gene was not expressed at day 3, but increased slowly at day 5, displaying four times the increment of basal expression at day 7. The gene discussed is TGFB1; the disease is keratoconus.