In the present study, HF diet-induced IL-1β release from adipose tissue or adipose tissue preadipocytes was markedly suppressed by NLRP3 inflammasome inhibitor MCC950 or endogenous n-3 PUFAs, suggesting that endogenous n-3 PUFAs repress IL-1β secretion via inhibiting NLRP3 inflammasome activation. This evidence concerns the gene NLRP3 and hydrops fetalis.