AKT1 and hydrops fetalis: To confirm whether reduced GSK-3β phosphorylation in vivo by HF diet can be restored by endogenous n-3 PUFA-mediated Akt phosphorylation in adipose tissue from Fat-1 HF mice, 3T3-L1 preadipocytes cultured in vitro were treated with PA or DHA alone or in combination with PI3K inhibitor alpelisib.