NLRP3 and hydrops fetalis: Here we demonstrated that the HF diet induced significantly elevated ROS in WT mice compared with fat-1 mice (Figure 5C), similar results were also observed in PA and/or DHA-treated 3T3-L1 preadipcytes (Figure 7F), suggesting that HF diet-induced NLRP3 inflammasome activation is tightly controlled by HF diet-induced ROS.