Hence, the expression levels of key proteins, i.e., Akt and p-Akt, mTOR and p-mTOR, PI3K and p-PI3K and cell proliferation marker, c-Myc were monitored in the A549 lung cancer cells treated with various concentrations of 24MD (0–100 μM) for 24 h. This evidence concerns the gene AKT1 and lung carcinoma.