These results are in agreement with those obtained in the present study, in that we did not find any significant differences in the thickness of the mucus layer among the groups, and a decrease in the protein expression of Claudin-1, observed in the group with induced obesity, restored the values to those comparable to the standard in the HFD + BCP group. Here, CLDN1 is linked to obesity due to melanocortin 4 receptor deficiency.