The subjects were divided into three groups based on the presence of SSc-specific, SSc-overlap, or no SSc-related antibodies, where SSc-specific antibodies were considered anti-centromere, anti-topoisomerase-I, anti-RNA-polymerase-III, anti-Th/To, and anti-fibrillarin, and the antibodies indicative of SSc-overlap were anti-PM/Scl, anti-Ku, and anti-U1RNP antibodies. The gene discussed is FBL; the disease is systemic sclerosis.