NFKB1 and pulmonary arterial hypertension: Similarly, to confirm the impact of miR-486-5p inhibitor and miR-26a-5p mimic on NF-κB signaling, hPAECs were first incubated with either PAH- or HC- EVs followed by transfection of miR inhibitor or mimic along with co-transfection of luciferase reporter or its negative control that contained multiple NF-κB binding sites in its promoter.