Compared with the HC EVs, PAH EVs-incubated hPAECs displayed a marked increase in NF-κB promoter activity measured in terms of luciferase intensity, which was significantly restricted in response to miR-486-5p-inhibitor and miR-26a-5p mimic transfection to PAH EVs exposed hPAECs (Figure 5D). Here, NFKB1 is linked to pulmonary arterial hypertension.