Overall, our results put forward a very variable presence of surface CXCR4 and ACKR3 across different BC cell lines and complex patterns of CXCR4/ACKR3 distribution and co-localization within cell lines, suggesting that the response of BC cells to CXCR4/ACKR3 ligands might be very heterogenous. Here, ACKR3 is linked to breast cancer.