SH2D3C and infection: The H1299 cells infected with wild-type (rIBV-WT) and rIBV-HA-3541 were harvested at 20 h post-infection and analyzed by Western blot with anti-HA antibodies, showing efficient detection of the HA-tagged nsp3 in cells infected with rIBV-HA-3541, but not in cells infected with rIBV-WT (Figure 2c).